ABSTRACT
Typhonium flagelliforme plant is an Indonesian herbal plant known as rodent tuber. Rodent tuber superior mutants have produced to increase the bioactive compound and more potential as anticancer than the mother plant. The purpose of this study was to determine the anticancer activity of ethanol extract, ethyl acetate fraction, and water fraction from the ethanol extract of rodent tuber superior mutant against MCF-7 breast cancer cells. Anticancer activity of ethanol extract, ethyl acetate fraction, and water fraction was carried out using the PrestoBlue assay method with multilevel concentration. The inhibitory concentration (IC50) values of ethanol extract, ethyl acetate fraction, and water fraction were respectively obtained for 1.60, 1.09, and-572.14 µg mL-1. Based on the results of the IC50 value, the water fraction does not have an anticancer activity or inhibits cancer cell proliferation of MCF-7. Rodent tuber superior mutant extract was tested to normal cells CV1 has an IC50 value of-81.72 µg mL-1 from these results there was no inhibition of growth of normal cells CV1. The cytotoxicity in ethyl acetate fraction is stronger than ethanol extract. Thus, the ethyl acetate fraction can be developed as promising agents as an anticancer treatment.
