{"id":4590,"date":"2024-08-12T11:37:26","date_gmt":"2024-08-12T04:37:26","guid":{"rendered":"https:\/\/research.binus.ac.id\/bdsrc\/?p=4590"},"modified":"2024-08-12T11:37:52","modified_gmt":"2024-08-12T04:37:52","slug":"primer-design-for-cloning-of-l-arabinose-isomerase-gene-from-arthrobacter-psychrolactophillus-into-plasmid-pet28a","status":"publish","type":"post","link":"https:\/\/research.binus.ac.id\/bdsrc\/2024\/08\/12\/primer-design-for-cloning-of-l-arabinose-isomerase-gene-from-arthrobacter-psychrolactophillus-into-plasmid-pet28a\/","title":{"rendered":"Primer Design for Cloning of L-arabinose isomerase Gene from Arthrobacter psychrolactophillus into plasmid pET28a(+)"},"content":{"rendered":"<p style=\"text-align: justify\">A novel putative L-arabinose isomerase (L-AI) called ApL-AI with the accession number WP_110486392.1 was successfully retrieved from Arthrobacter psychrolactophilus strain B7 genome (Accession: NZ_QJVC01000021.1) trough genome mining analysis. The primers pair designed in this study successfully amplified the gene using 60 \u00b0C of PCR annealing temperature and supported the gene amplicon to insert into the pET28a(+) to form plasmid pET28a(+)-ApLAI. It was proved by the appearance of a 1557-bp amplification band on the gel electrophoresis. The sequencing analysis also revealed that the gene was inserted in the correct direction, with the gene positioned after the promoter and finished with a terminator. Therefore, the plasmid can be used to express the ApL-AI gene to produce the ApL-AI enzyme for downstream analysis and further prospecting.<\/p>\n<p style=\"text-align: justify\"><strong>Authors:<\/strong><br \/>\n<span data-sheets-root=\"1\">Rudi Nirwantono, David Herawan, Salsabila Firdausa, Issa Nuryana, Fina Amreta Laksmi, Bens Pardamean<\/span><\/p>\n<p style=\"text-align: justify\"><em>7th International Conference on Eco Engineering Development<\/em><\/p>\n<p style=\"text-align: justify\"><a href=\"https:\/\/www.researchgate.net\/publication\/375747280_Primer_Design_for_Cloning_of_L-arabinose_isomerase_Gene_from_Arthrobacter_psychrolactophillus_into_plasmid_pET28a\" target=\"_blank\" rel=\"noopener\">Read Full Article<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A novel putative L-arabinose isomerase (L-AI) called ApL-AI with the accession number WP_110486392.1 was successfully retrieved from Arthrobacter psychrolactophilus strain B7 genome (Accession: NZ_QJVC01000021.1) trough genome mining analysis. The primers pair designed in this study successfully amplified the gene using 60 \u00b0C of PCR annealing temperature and supported the gene amplicon to insert into the [&hellip;]<\/p>\n","protected":false},"author":7,"featured_media":4594,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[12],"tags":[],"class_list":["post-4590","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications"],"_links":{"self":[{"href":"https:\/\/research.binus.ac.id\/bdsrc\/wp-json\/wp\/v2\/posts\/4590","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.binus.ac.id\/bdsrc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/research.binus.ac.id\/bdsrc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/research.binus.ac.id\/bdsrc\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/research.binus.ac.id\/bdsrc\/wp-json\/wp\/v2\/comments?post=4590"}],"version-history":[{"count":1,"href":"https:\/\/research.binus.ac.id\/bdsrc\/wp-json\/wp\/v2\/posts\/4590\/revisions"}],"predecessor-version":[{"id":4593,"href":"https:\/\/research.binus.ac.id\/bdsrc\/wp-json\/wp\/v2\/posts\/4590\/revisions\/4593"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/research.binus.ac.id\/bdsrc\/wp-json\/wp\/v2\/media\/4594"}],"wp:attachment":[{"href":"https:\/\/research.binus.ac.id\/bdsrc\/wp-json\/wp\/v2\/media?parent=4590"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/research.binus.ac.id\/bdsrc\/wp-json\/wp\/v2\/categories?post=4590"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/research.binus.ac.id\/bdsrc\/wp-json\/wp\/v2\/tags?post=4590"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}